C146-E243 Technical Report: Determination of Fatty-acid Composition of Lipids in Human Embryonic Stem Cells Using GC-MS
نویسندگان
چکیده
Lipids typically exist in a state in which various fatty acids are bound to a backbone such as glycerol, sphingosine, cholesterol, etc. With recent advances in analytical techniques, especially those associated with mass spectrometry, identification of lipid components, including their molecular species, is becoming possible. However, it is still difficult to identify all the components of lipids. Fatty acids, as mentioned above, are a major component of lipids, and obtaining information on the fatty acid composition of all the lipids contained in a biological sample is a critical step in comprehensive lipid analysis. An effective approach to analyzing fatty acid content is to apply gas chromatography/mass spectrometry (GC/MS) to the analysis of the constituent fatty acids of derivatized methyl esters.
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